| Accession NO. | GSE144113 |
|---|---|
| Cell Count | |
| Technique | |
| PMID | |
| Title | Transcriptome profile of drug resistant metaplastic breast cancer cell lines |
| Organism | Homo sapiens |
| Overall design | Cells were treated with increasing concentrations of drug until they were able to survive and proliferate after a pulse of cytotoxic drug. HS-TX: HS578T cells, 100 nM paclitaxel, 5 days pulse; HS-DOX: HS-578T cells, 100 nM doxorubicin, 3 days pulse; BAS-TX: BAS cells, 20 nM paclitaxe, 3 days pulse; BAS-DOX, BAS cells, 160 nM doxorubicin, 3 days pulse. For the array experiment, RNA isolated from three (drug resistant cells) or four (parental naive cells) independent cultures were used for each condition. |
| Summary | Two metaplastic breast cancer cell lines were used: a widely available, HS578T, and a novel line isolated from a metaplastic breast cancer tumor, BAS. Doxorubicin and paclitaxel resistant derivatives of these lines were generated and transcriptome profiling performed. |
A spatial transcription database site for diseases of human systems
